Sample preparation of mango leaf extract

Accurately weigh 400g Mangifera Indica Extract, add 8 times the amount of 70% ethanol solvent, soak for 1 hour, boil and reflux for 2 hours, filter while hot, add 8 times the amount of 70% ethanol solvent to the filter residue, continue to reflux for 2 hours, combine the filtrate for 2 times, put it at room temperature, accurately measure the volume of the extract solution to a total of 6.86l, and concentrate to 800ml according to the ratio of crude drug (Gardenia Yellow): solution volume (ML) = 1:2 , 3000 R / min, centrifugation for 15 min to discard the precipitate. Accurately measure 800ml of D101 macroporous adsorption resin pretreated, and wet column.

Take the sample of the treated concentrated solution, the Astaxanthin Supplier is 40min / BV, pre elute with 3bv water, elute with 5 times of 15% ethanol, recover ethanol from 15% ethanol eluent and concentrate to the end, weigh 10g of mangiferin crude product, heat and dissolve with 20ml of 50% ethanol solution to make the solution supersaturated, stand, cool and filter, scrape off the filter cake, wash the sediment with 20ml of 50% ethanol solution, mix well Evenly, filter again, put the filter cake into the vacuum drying oven for drying at 60 ℃, and obtain the Mango Leaf Extract sample.